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Image Search Results
Journal: eLife
Article Title: The LRRK2 G2019S mutation alters astrocyte-to-neuron communication via extracellular vesicles and induces neuron atrophy in a human iPSC-derived model of Parkinson’s disease
doi: 10.7554/eLife.73062
Figure Lengend Snippet:
Article Snippet: Antibody ,
Techniques: Magnetic Beads, Knock-In, Labeling, Purification, SNP Genotyping Assay, Recombinant, Sequencing, Software
Journal: Frontiers in Neurology
Article Title: In Vitro Differentiation of Bone Marrow Mesenchymal Stem Cells into Neuron-Like Cells by Cerebrospinal Fluid Improves Motor Function of Middle Cerebral Artery Occlusion Rats
doi: 10.3389/fneur.2016.00183
Figure Lengend Snippet: Characteristics of hCSF-induced rat BMSC-differentiated neuron-like cells . (A,B) show post-induction by hCSF at day 1 and 4 (40×). Scale bar = 100 μm. (C) The protein levels were measured by a GELDOC instrument and normalized with respect to β-actin, which was chosen as an internal control. Each experiment was repeated at least three times. Variations in protein expression are given as arbitrary units. The expression of NES in cells induced by hCSF (0.43 ± 0.07) was significantly higher than that of P3-generation BMSCs (0.09 ± 0.03); meanwhile, GFAP also had small amount of expression (0.09 ± 0.02). However, P3-generation of BMSCs almost did not express GFAP (0 ± 0). (D–F) show Cells were positive for both NSE and GFAP, but expression levels for NSE were higher than GFAP. Western blot analysis of NSE and GFAP protein expression (200×). Scale bar = 100 μm.
Article Snippet: The slices were stained alternatively for neuronal specific enolase (NSE, neuronal marker) or rabbit anti-rats glial fibrillary acidic protein (GFAP, a astrocyte marker), mouse anti-BrdU antibody (1:100), rabbit anti-rats NSE antibody (1:200; Wuhan Boster Biological Technology, Ltd., China) or
Techniques: Control, Expressing, Western Blot
Journal: Frontiers in Neurology
Article Title: In Vitro Differentiation of Bone Marrow Mesenchymal Stem Cells into Neuron-Like Cells by Cerebrospinal Fluid Improves Motor Function of Middle Cerebral Artery Occlusion Rats
doi: 10.3389/fneur.2016.00183
Figure Lengend Snippet: Distribution of transplanted cells in the ischemic core of the brain on day 32 after transplantation in rats subjected to MCAO . Green labeling indicates BrdU-positive cells. DAPI (blue) labels nuclei. Immunofluorescent staining showing BrdU-positive transplanted cells (green) and NSE-positive cells (or GFAP-positive cells) (red). BrdU/NSE-positive double-labeled cells (or BrdU/GFAP-positive double-labeled cells) (yellow) (A,B) BMSC-N group; (C,D) BMSC group. The number of BrdU/GFAP-positive double-labeled cells or BrdU/NSE-positive double-labeled cells in the BMSC-N group was higher than that in the BMSC group. The BMSC-N group showed more BrdU/NSE-positive double-labeled cells than BrdU/GFAP-positive double-labeled cells. Conversely, the BMSC group had more BrdU/GFAP-positive double-labeled cells than BrdU/NSE-positive double-labeled cells. Significance levels are as shown in Table . Scale bar = 100 μm.
Article Snippet: The slices were stained alternatively for neuronal specific enolase (NSE, neuronal marker) or rabbit anti-rats glial fibrillary acidic protein (GFAP, a astrocyte marker), mouse anti-BrdU antibody (1:100), rabbit anti-rats NSE antibody (1:200; Wuhan Boster Biological Technology, Ltd., China) or
Techniques: Transplantation Assay, Labeling, Staining
Journal: Frontiers in Neurology
Article Title: In Vitro Differentiation of Bone Marrow Mesenchymal Stem Cells into Neuron-Like Cells by Cerebrospinal Fluid Improves Motor Function of Middle Cerebral Artery Occlusion Rats
doi: 10.3389/fneur.2016.00183
Figure Lengend Snippet: Effects of BMSC-Ns and BMSCs transplantation on neurological performance, BrdU labeling, and cell differentiation (data represent mean ± SD, n = 8 per group): comparison of the antigen differentiation ratios of neural cells after cell implantation .
Article Snippet: The slices were stained alternatively for neuronal specific enolase (NSE, neuronal marker) or rabbit anti-rats glial fibrillary acidic protein (GFAP, a astrocyte marker), mouse anti-BrdU antibody (1:100), rabbit anti-rats NSE antibody (1:200; Wuhan Boster Biological Technology, Ltd., China) or
Techniques: Transplantation Assay, Labeling, Cell Differentiation, Comparison, Control